TO STUDY VARIOUS NEUROLOGICAL ACTION OF FRE AS ANALGESIC USING WRITHING METHOD (CHEMICAL INDUCED PAIN) IN ALBINO MICE

Abstract

Method: For the preparation of extract 100gm of dried coarse powdered leaves were charged in to the soxhlet’s apparatus (hot extraction) and extracted successively with chloroform. The successive chloroform extract (deep brown colour) was filtered & dried under reduced pressure to get a solid mass free from the solvent. The crude extract thus obtained was further fractionated. The solvent fractionation was done with alcohol and acetone.

Result: FRE at a dose of 50mg/kg did not show a significant analgesic activity as compared to control group (P< 0.05). At the dose of 100mg/kg it showed a highly significant analgesic activity as compare to control group (P< 0.01). At the dose of 200 mg/kg it showed a highly significant decrease in number of writhes as compared to control as well as diclofenac treated group (P<0.01). FRE at a dose of 200mg/kg showed 66.21 % inhibition in number of writhes as compared to 50.02 % inhibition by diclofenac.

Conclusion: The present study was conducted at Dept. of Pharmacology, Sri Aurobindo Institute of Medical Sciences & Research Centre, Indore FRE endowed with both central and peripheral analgesic properties in dose dependent manner. It also enhances the analgesic effect of the standard drugs.

Study Designed: Observational Study.

Keywords: FRE, Analgesic, Albino mice, Neurological & Writhing Method.