COMPOSING THE MIXTURE OF A.CORDIFOLIA LEAF EXTRACT IN ELIMINATING SALMONELLA TYPHI AND STAPHYLOCOCCUS AUREUS BACTERIA

Abstract

Background: A.cordifolia leaf is one of the traditional medicinal plants that have many benefits, including cough medicine, diabetes, shortness of breath, burns, post-operative wounds, dysentery, kidney inflammation, inflammation of the intestine, acne, bleeding gums, smoothing menstruation, increase appetite, and increase endurance. This research aims to find out the minimum concentration of A.cordifolia leaf extract that was able to kill bacteria Salmonella typhi

Methods: This research is an experimental laboratory study with a research design using a Completely Randomized Design (CRD) in which the treatment of test bacteria is carried out with variations in concentration and contact time of the A.cordifolia leaf extract and A.cordifolia leaf using alcohol extract. The controlled variable was the concentration of A.cordifolia  leaf extract with contact time variation while the response variable was the result of bacterial colony growth. For negative control applied sterilized aqua (0%) and as a comparison chloramphenicol was used.

Result: The results showed that A.cordifolia which extracted by ethanol solvent killed bacteria S. Typhi at a concentration of 80% with a contact time of 10 minutes, and bacteria S. aureus at a level of 100% with a contact time of 10 minutes, whereas A.cordifolia water extract could kill bacteria S.typhi at a concentration 80% with a contact time of 20 minutes, and bacteria S. aureus at a concentration of 100% with a contact time of 20 minutes.

Conclusion: A.cordifolia, which extracted by ethanol solvent, has a stronger killing power than one extracted with water solvent.

Keyword: A.cordifolia leaves, Salmonella typhi, Staphylococcus aureus, extraction